smooth the muscle cells in the middle layer of the uterine wall, which is important for maintaining pregnancyĪ person may be familiar with hCG because it is the hormone that pregnancy tests check for in urine.promote the development of new blood vessels in the uterus.To maintain a pregnancy, hCG triggers the body to produce another hormone, progesterone. But it is usually associated with pregnancy because it plays a key role. This naturally occurring hormone is present in males and females. Share on Pinterest A doctor may measure a person’s hCG level during pregnancy to identify any underlying health problems. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 81. also provides other Autoimmune Disease ELISA Kits. For additional details please refer to the instructions for use.ĭiagnostic Automation Inc. If antigen is present in the specimen, it will combine with the antibody on the well. then serum is added to the hCG antibody coated microtiterwells and incubated with the Zero Buffer. The assay system utilizes one anti- hCG antibody for microtiter wells immobilization and another mouse monoclonal anti-bhCG antibody in the antibody-enzyme horseradish peroxidase conjugate solution. The hCG Quantitative Test Kit is based on a solid phase enzyme-linked immunosorbent assay. The antibodies developed for the test will determine a minimal concentration of 2 mIU/ml. The HCG EIA test provides a rapid, sensitive and reliable assay. Elevated levels of HCG have also been detected in serum from patients with abnormal physiological conditions not related to pregnancy.
With the availability of sensitive quantitative assays for the measurement of serum HCG, it has been shown that HCG levels can be useful in predicting spontaneous abortions, aiding in the detection of ectopic pregnancy and multiple gestation. The placental hormone, HCG, is similar to luteinizing hormone (LH), follicle stimulating hormone (FSH), and human thyroid stimulating hormone (HTSH). The subunits are cleared rapidly and excreted by the kidney. HCG circulates as the intact molecule in the serum of normal women who have an uncomplicated pregnancy. As uncomplicated pregnancies progress, the serum HCG levels increase to a peak concentration, then decrease and plateau. The rapid rise in HCG serum levels after conception makes it an excellent marker for early confirmation and monitoring of pregnancy. Human chorionic gonadotropin (HCG) is a sialoglycoprotein initially secreted by the trophoblastic cells of the placenta shortly after implantation of the fertilized ovum into the uterine wall. EIA kit Microplate Reader with a 450 nmīeta hCG (Total) ELISA Kit Background Information:
Material Provided with Beta hCG (Total) ELISA Kit:ġ.Beta hCG EIA Test Antibody-coated microtiter wellsĢ.Reference standards,0,5,20,50,150,300 mIU/ml, liquid standardĤ.Beta hCG Elisa Kit Enzyme Conjugate Reagentħ.Beta hCG EIA Kit Wash Buffer Concentrate 50XĤ. The Diagnostic Automation b-hCG EIA test kit is mainly intended to quantitatively determine Total beta-hCG concentration in human serum.